Contrast is the difference in light intensity between the image and the adjacent background, making details visible under a microscope. It is crucial for distinguishing structures in microscopic samples.
5 Must Know Facts For Your Next Test
Higher contrast improves the visibility of fine details in a sample.
Staining techniques are often used to increase contrast in biological specimens.
Phase-contrast microscopy enhances contrast without staining by utilizing differences in refractive index.
Dark-field microscopy increases contrast by illuminating the specimen with light that does not directly enter the objective lens.
Fluorescence microscopy uses fluorescent dyes to create high-contrast images based on emitted light.