Chromophores

Definition

Chromophores are molecules or parts of molecules responsible for the color of compounds due to their ability to absorb certain wavelengths of light. In microscopy, they are crucial for staining and visualizing specimens.

5 Must Know Facts For Your Next Test

1. Chromophores absorb light at specific wavelengths, which makes them visible under a microscope.
2. They are integral in various staining techniques used in microbiology to differentiate between types of microorganisms.
3. Common chromophores include dyes such as crystal violet and safranin used in Gram staining.
4. The absorption spectrum of a chromophore is determined by its chemical structure and environment.
5. Chromophores can be naturally occurring within cells (endogenous) or introduced externally through stains (exogenous).

Review Questions

• What role do chromophores play in microscopy?
• Name two common dyes that act as chromophores in Gram staining.
• How does the chemical structure of a chromophore affect its function?

Related terms

Staining Techniques: Methods used to enhance contrast in microscopic specimens using dyes.

Absorption Spectrum: Range of wavelengths absorbed by a substance, indicating its interaction with light.

Gram Staining:

A differential staining technique that categorizes bacteria into Gram-positive and Gram-negative based on cell wall properties.