Autoclave

Definition

An autoclave is a device that uses steam under high pressure to sterilize equipment and materials. It is commonly used in microbiology labs to eliminate all forms of microbial life, including spores.

5 Must Know Facts For Your Next Test

1. Autoclaves typically operate at 121°C and 15 psi for a minimum of 15 minutes.
2. Sterilization via autoclaving is effective against bacteria, viruses, fungi, and spores.
3. Indicators such as autoclave tape are used to confirm successful sterilization cycles.
4. Items that cannot withstand high heat or moisture should not be autoclaved.
5. Regular maintenance and validation of autoclaves are essential to ensure their effectiveness.

Review Questions

• What temperature and pressure conditions are standard for operating an autoclave?
• Why are indicators like autoclave tape important when using an autoclave?
• What types of items should not be sterilized using an autoclave?

Related terms

Sterilization:

The process of eliminating all forms of microbial life.

Pasteurization:

A method of reducing microbial numbers in food and beverages through controlled heating.

Disinfection:

The process of eliminating most pathogenic microorganisms on inanimate objects, but not necessarily all microbes or spores.