Northern blotting

Definition

Northern blotting is a laboratory technique used to detect specific RNA sequences in a sample. It involves the separation of RNA by gel electrophoresis, transfer to a membrane, and hybridization with a labeled probe.

5 Must Know Facts For Your Next Test

1. Northern blotting specifically detects RNA molecules.
2. The process involves gel electrophoresis, membrane transfer, and hybridization with a probe.
3. It is commonly used to study gene expression levels.
4. Radioactive or non-radioactive probes can be used for detection.
5. Northern blotting can provide information about the size and abundance of mRNA transcripts.

Review Questions

• What type of molecule does Northern blotting detect?
• Describe the three main steps involved in Northern blotting.
• Why is Northern blotting useful in studying gene expression?

Related terms

Southern Blotting: A technique used to detect specific DNA sequences in a sample.

Western Blotting: A method for detecting specific proteins in a sample using antibodies.

Hybridization Probe: A fragment of DNA or RNA used to detect complementary sequences by binding through hybridization.